Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10769066 | Biochemical and Biophysical Research Communications | 2005 | 5 Pages |
Abstract
We have developed a novel nanoparticulate luminescent probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This construct may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Release of AuNPs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47Â h of exposure to 0.2Â mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.
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Authors
Emmanuel Chang, Jordan S. Miller, Jiantang Sun, William W. Yu, Vicki L. Colvin, Rebekah Drezek, Jennifer L. West,