Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10769262 | Biochemical and Biophysical Research Communications | 2005 | 5 Pages |
Abstract
By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 107 cells; O2 consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n = 3 for each cell line). Both types of synoviocytes showed a high O2 consumption, which was slowered after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O2 consumption, unchanged by anoxia, and no free radical production. These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis.
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Authors
Nicole Schneider, Ange L. Mouithys-Mickalad, Jean-Philippe Lejeune, Ginette P. Deby-Dupont, Maryse Hoebeke, Didier A. Serteyn,