Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10769957 | Biochemical and Biophysical Research Communications | 2005 | 5 Pages |
Abstract
SID-1 is a transmembrane protein that mediates systemic RNA interference in Caenorhabditis elegans. Here we show that the mammalian SID-1 homologue FLJ20174 localizes to the cell membrane of human cells and enhances their uptake of small interfering RNA (siRNA), resulting in increased siRNA-mediated gene silencing efficacy. This is the first demonstration to show that overexpression of a membrane protein enhances siRNA internalization in mammalian cells. This observation raises the possibility of enhancing the efficacy of RNA interference.
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Authors
Mark S. Duxbury, Stanley W. Ashley, Edward E. Whang,