Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10769961 | Biochemical and Biophysical Research Communications | 2005 | 5 Pages |
Abstract
Firefly luciferase is able to convert l-luciferin into luciferyl-CoA even under ordinary aerobic luciferin-luciferase reaction conditions. The luciferase is able to recognize strictly the chirality of the luciferin structure, serving as the acyl-CoA synthetase for l-luciferin, whereas d-luciferin is used for the bioluminescence reaction. d-Luciferin inhibits the luciferyl-CoA synthetase activity of l-luciferin, whereas l-luciferin retards the bioluminescence reaction of d-luciferin, meaning that both enzyme activities are prevented by the enantiomer of its own substrate.
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Authors
Mitsuhiro Nakamura, Shojiro Maki, Yoshiharu Amano, Yutaka Ohkita, Kazuki Niwa, Takashi Hirano, Yoshihiro Ohmiya, Haruki Niwa,