Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770058 | Biochemical and Biophysical Research Communications | 2005 | 6 Pages |
Abstract
We have developed a quantitative assay of calmodulin (CaM) binding to S-Tag labeled peptides derived from G-protein coupled receptor (GPCR) sequences. CaM binding of peptides derived from the third intracellular loop (i3) of μ opioid receptor (MOR) was confirmed and the CaM-binding motif refined. A MORi3 peptide with a Lys > Ala substitution-shown to reduce CaM-binding of intact MOR-bound fivefold less avidly than the wild-type peptide. Screening peptides derived from i3 loops of other GPCR families confirmed 5HT1A, and identified muscarinic receptor 3, and melanocortin receptor 1, as proteins carrying CaM-binding domains. The use of S-Tag labeling can serve for rapid screening of putative CaM-binding domains in GPCRs.
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Authors
Ying Zhang, Danxin Wang, Wolfgang Sadée,