Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770265 | Biochemical and Biophysical Research Communications | 2005 | 6 Pages |
Abstract
Insulin may be a potential target of ONOOâ during conditions involving accelerated rates of oxygen radical and nitric oxide generation in pancreatic islet β-cells. To evaluate the effect of protein tyrosine nitration on insulin function, the porcine insulin was nitrated by ONOOâ. Insulin nitration was confirmed by spectral changes, Native-PAGE, and mass spectrometry. The bioassay results show that mono-nitro-insulin affected its receptor binding and hypoglycemic capacities to a certain extent, but still hold high biological activities.
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Authors
Quan Chi, Tielin Wang, Kaixun Huang,