Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770348 | Biochemical and Biophysical Research Communications | 2005 | 9 Pages |
Abstract
Cellular adhesion plays important roles in a variety of biological processes. The ADAM family contains disintegrin-like and metalloproteinase-like domains which potentially have cell adhesion and protease activities. Recent studies suggest that the interaction between 14-3-3ζ and ADAM22cyt can regulate cell adhesion and spreading, therefore it has a potential role in neural development and function. 14-3-3 family has seven highly conserved members that regulate various cellular functions. Using yeast two-hybrid method, we identified that ADAM22cyt bound some other 14-3-3 family members. The interaction was further confirmed by in vitro protein pull-down assay and co-immunoprecipitation. We also found that the overexpression of exogenous ADAM22 in HEK293 cells could significantly enhance cell adhesion and spreading, compared with the truncated ADAM22 lack of 14-3-3 binding motifs. These results strongly demonstrated a functional role for ADAM22/14-3-3 in cell adhesion and spreading.
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Authors
Pengcheng Zhu, Yingying Sang, Huanbing Xu, Jing Zhao, Rener Xu, Yubo Sun, Tianhong Xu, Xiaolei Wang, Linfeng Chen, Hanping Feng, Changben Li, Shouyuan Zhao,