Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770433 | Biochemical and Biophysical Research Communications | 2005 | 10 Pages |
Abstract
The full length genes encoding the catalytic α and glycosylated β subunits of the sodium pump (Na+-K+-ATPase) were cloned and characterized from silver sea bream gill. Using in vitro preparations of gill tissue it was found that growth hormone (10 and 100 ng/ml) caused an increase in subunit transcription, translation, and Na+-K+-ATPase enzyme activity. Similarly, insulin-like growth factor 1 (10 and 100 ng/ml) also caused an increase in Na+-K+-ATPase subunit amounts and enzyme activity. Cortisol (10 and 100 ng/ml) increased α subunit transcript and protein but did not modulate β subunit expression or enzyme activity. Ovine prolactin did not cause any changes in Na+-K+-ATPase subunit transcription, translation or enzyme activity. This study is the first to describe how both Na+-K+-ATPase α and β subunits are modulated at transcriptional and translational levels in fish osmoregulatory tissue upon exposure to hormones.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Eddie E. Deane, Norman Y.S. Woo,