Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770497 | Biochemical and Biophysical Research Communications | 2005 | 8 Pages |
Abstract
Legionella pneumophila is a facultative intracellular human pathogen and an important cause of Legionnaires' disease, a severe form of pneumonia. Recently, we showed the presence of a putative twin-arginine translocation (Tat) pathway in L. pneumophila Philadelphia-1. This secretion pathway is used to transport completely folded proteins across the cytoplasmic membrane. The importance of the Tat pathway in L. pneumophila was investigated by constructing a tatB and a tatC mutant. Functionality of the Tat pathway was shown using a proven heterologous Tat substrate. It was shown that tatB and tatC are involved in intracellular replication in Acanthamoeba castellanii and differentiated U937 cells, and in biofilm forming ability. A putative Legionella Tat substrate was identified via 2D gel electrophoresis.
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Authors
Emmy De Buck, Liesbeth Maes, Eef Meyen, Lieve Van Mellaert, Nick Geukens, Jozef Anné, Elke Lammertyn,