Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770526 | Biochemical and Biophysical Research Communications | 2005 | 7 Pages |
Abstract
α-Tocopheryl succinate (α-TOS), a redox-inactive analog of vitamin E, induces cell cycle arrest, differentiation, and triggers apoptosis. We examined the ability of α-TOS to induce cytostasis and/or apoptosis in two human osteosarcoma cell lines, which carry wild-type pRb but differ in the p53 status. In the wt-p53 cells, α-TOS induced apoptosis, which was associated with p53 activation and enhanced E2F1 expression. Mutant p53 cells failed to undergo apoptosis when challenged with α-TOS. The cell growth arrest after α-TOS treatment was associated with a reduced expression of E2F1. Knocking down E2F1 rendered the α-TOS-sensitive cells rather resistant to the apoptotic stimulus inducing a marked and prolonged cell growth arrest. We conclude that α-TOS induces cell growth arrest or apoptosis involving E2F1.
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Authors
Renata Alleva, Maria Serena Benassi, Marco Tomasetti, Nina Gellert, Francesca Ponticelli, Battista Borghi, Piero Picci, Jiri Neuzil,