Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770743 | Biochemical and Biophysical Research Communications | 2005 | 8 Pages |
Abstract
The adhesion of highly activated neutrophils to cerebral microvascular endothelial cells (MVECs) may contribute to disruption and hyperpermeability of the blood-brain barrier (BBB) after cardiac surgery with prolonged cardiopulmonary bypass (CPB). A correlation between CPB duration and neutrophil-mediated BBB damage has not been investigated on the cellular level yet. Therefore, we studied the effects of neutrophils from cardiac surgery patients with CPB time <80 min (group I; n = 8) and >80 min (group II; n = 8) on the integrity of cultured porcine MVEC. Ex vivo, neutrophils of group II but not of group I significantly degraded the zonula adherens molecule β-catenin whereas VE-cadherin and occludin were not modified. The transendothelial electric resistance as a measure for the integrity of the endothelial monolayers was reduced over time in both groups. In conclusion, prolonged CPB time entails neutrophil-mediated decrease in MVEC β-catenin expression, and thus may be an important trigger for BBB disruption.
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Authors
Alina Maria Schuller, Joachim Windolf, Roman Blaheta, Jindrich Cinatl, Jörg Kreuter, Gerhard Wimmer-Greinecker, Anton Moritz, Martin Scholz,