Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770821 | Biochemical and Biophysical Research Communications | 2005 | 5 Pages |
Abstract
X-ray absorption spectroscopy at the sulfur K-edge (2.4-2.6Â keV) provides a sensitive and specific technique to identify S-nitroso compounds, which have significance in nitric oxide-based cell signaling. Unique spectral features clearly distinguish the S-nitroso-form of a cysteine residue from the sulfhydryl-form or from a methionine thioether. Comparison of the sulfur K-edge spectra of thiolate, thiol, thioether, and S-nitroso thiolate compounds indicates high sensitivity of energy positions and intensities of XAS pre-edge features as determined by the electronic environment of the sulfur absorber. A new experimental setup is being developed for reaching the in vivo concentration range of S-nitroso thiol levels in biological samples.
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Authors
Robert K. Szilagyi, David E. Schwab,