| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10770946 | Biochemical and Biophysical Research Communications | 2005 | 7 Pages |
Abstract
We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400Â GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.
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Authors
Joanna Lukomska, Ignacy Gryczynski, Joanna Malicka, Slawomir Makowiec, Joseph R. Lakowicz, Zygmunt Gryczynski,