Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10770982 | Biochemical and Biophysical Research Communications | 2005 | 6 Pages |
Abstract
Activated matrix metalloproteinases (MMPs) in patients with acute coronary syndromes may contribute to plaque destabilization. Since reactive oxygen species (ROS) induce MMP-2 and angiotensin II (ANG II) enhances NAD(P)H-oxidase-dependent ROS formation, we assessed whether ANG II induces MMP-2 in a NAD(P)H-oxidase-dependent manner. MMP-2 mRNA expression and activity were analyzed in wildtype and p47phox-deficient (p47phoxâ/â) murine smooth muscle cells (SMC). To address a clinical implication, sections of human atherosclerotic arteries were stained for MMP-2, p47phox, ANG II, AT1-receptor, and α-smooth muscle cell actin (α-SMC actin). MMP-2 protein expression and activity from these arteries were compared to those without atherosclerosis. ANG II enhances mRNA synthesis and activity of MMP-2 in a p47phox-dependent manner. Immunohistochemical analyses revealed a co-localization of MMP-2 with p47phox, ANG II, AT1-receptor, and α-SMC actin. MMP-2 protein expression and gelatinolytic activity are increased in atherosclerotic arteries. Thus, activation of the renin-angiotensin system may contribute to plaque destabilization via ROS-dependent induction of MMP-2.
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Authors
Maren Luchtefeld, Karsten Grote, Christina Grothusen, Sylvia Bley, Nele Bandlow, Tina Selle, Martin Strüber, Axel Haverich, Udo Bavendiek, Helmut Drexler, Bernhard Schieffer,