Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10771145 | Biochemical and Biophysical Research Communications | 2005 | 6 Pages |
Abstract
Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Chao Qi, Yiwei Tony Zhu, Jeffrey Chang, Anjana V. Yeldandi, M. Sambasiva Rao, Yi-Jun Zhu,