| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10771169 | Biochemical and Biophysical Research Communications | 2005 | 4 Pages |
Abstract
Some reports showed that serotonergic system might have existed and that 5-hydroxytryptamine (5-HT) was detected in the hamster heart. The source of 5-HT in the heart, however, remains to be fully elucidated. So the present study was designed to define serotonergic system and to clarify which cell could produce 5-HT in the heart. As a result, 5-HT was detected in homogenates of HL-1 cardiomyocytes by high performance liquid chromatography with fluorescence detection, but not in those of neonatal rat non-cardiomyocytes (NMCs). And TPH and AADC mRNAs were expressed in HL-1 cardiomyocytes and neonatal rat cardiomyocytes (MCs), not in NMCs. mRNAs of 5-HT2A receptor were detected in both MCs and NMCs, and those of 5-HT2B receptor in NMCs. These findings definitively demonstrate that 5-HT is secreted from the myocytes of the heart and strongly implied that 5-HT might play a certain role in cardiac physiology.
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Authors
Keiichi Ikeda, Katsuyoshi Tojo, Chikara Otsubo, Takashi Udagawa, Kensuke Kumazawa, Masahiro Ishikawa, Goro Tokudome, Tatsuo Hosoya, Naoko Tajima, William C. Claycomb, Kazuwa Nakao, Masahiro Kawamura,