Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10771262 | Biochemical and Biophysical Research Communications | 2005 | 6 Pages |
Abstract
The serotonin1A (5-HT1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors. We report here that solubilization of the hippocampal 5-HT1A receptor by the zwitterionic detergent CHAPS is accompanied by loss of membrane cholesterol which results in a reduction in specific agonist binding activity and extent of G-protein coupling. Importantly, replenishment of cholesterol to solubilized membranes using MβCD-cholesterol complex restores the cholesterol content of the membrane and significantly enhances the specific agonist binding activity and G-protein coupling. These novel results provide useful information on the role of cholesterol in solubilization of G-protein-coupled receptors, an important step for molecular characterization of these receptors.
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Authors
Amitabha Chattopadhyay, Md. Jafurulla, Shanti Kalipatnapu, Thomas J. Pucadyil, Kaleeckal G. Harikumar,