Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10771305 | Biochemical and Biophysical Research Communications | 2005 | 9 Pages |
Abstract
Progenitor cells exist in the adult pancreas and transform to endocrine cells in pathological conditions. To address the mechanism of β cell regeneration, mice were treated with streptozotocin (STZ group) or streptozotocin and exendin-4 (STZ + Ex-4 group), and the expression of PDX-1, Ngn3, insulin, IRS-2, and Foxo1 was investigated. PDX-1 mRNA was upregulated biphasically and induction of Ngn3 mRNA occurred shortly after the first increase of PDX-1 expression, a pattern similar to that observed during embryogenesis. PDX-1-positive cells appeared only in islet-like cell clusters (ICCs) in STZ group, but they appeared both in ducts and ICCs in STZ + Ex-4 group. Ngn3-positive cells emerged in ICCs but not in ducts. Therefore, regeneration seemed to occur mainly from intra-islet stem/progenitor cells. Exendin-4 upregulated PDX-1 expression which paralleled increased IRS-2 expression and translocation of Foxo1 from nucleus to cytoplasm. Further analysis of β cell regeneration should help in the design of novel therapy for diabetes.
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Authors
Shoko Kodama, Tetsushi Toyonaga, Tatsuya Kondo, Kazuya Matsumoto, Kaku Tsuruzoe, Junji Kawashima, Hideo Goto, Kazuhiko Kume, Shoen Kume, Michiharu Sakakida, Eiichi Araki,