Article ID Journal Published Year Pages File Type
10771346 Biochemical and Biophysical Research Communications 2005 5 Pages PDF
Abstract
Methyl mercaptan is derived from l-methionine by the action of l-methionine-α-deamino-γ-mercaptomethane lyase (METase) and is a major component of oral malodor. This compound is highly toxic and is thought to play an important role in periodontal disease. We found that Treponema denticola, a member of the subgingival biofilm at periodontal disease sites, produced a large amount of methyl mercaptan even at low concentration of l-methionine. METase activity in a cell-free extract from T. denticola was detected by two-dimensional electrophoresis under non-denaturing conditions, and the protein spot that exhibited high METase activity was identified using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The identified gene produced a METase with a Km value for l-methionine (0.55 mM) that is much lower than those of METases previously identified in the other organisms. This result suggests that T. denticola is an important producer of methyl mercaptan in the subgingival biofilm.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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