Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10771872 | Biochemical and Biophysical Research Communications | 2005 | 6 Pages |
Abstract
Transforming growth factor-β1 (TGF-β1) is a key factor in a variety of physiological and pathological processes. Vascular endothelial growth factor (VEGF) is a key angiogenic factor, and vascular change is one of the features of airway remodeling. We examined the effect of TGF-β1 on VEGF production by fibroblasts from mice lacking expression of Smad2 or Smad3 as well as human lung fibroblasts treated with or without Smad2 or Smad3 siRNA. TGF-β1 stimulated VEGF production by fibroblasts from Smad2 deficient animals and wildtype animals. In contrast, TGF-β1 did not affect VEGF production by fibroblasts from Samd3 deficient mice. Similarly, TGF-β1 failed to stimulate VEGF production by HFL-1 cells treated with Samd3 siRNA but significantly increased VEGF production by the cells treated with Smad2 siRNA. These result suggest that TGF-β1 stimulation of VEGF production by fibroblasts is regulated by Smad3 but not by Smad2 signaling.
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Authors
Tetsu Kobayashi, Xiangde Liu, Fu-Qiang Wen, Qiuhong Fang, Shinji Abe, Xiang Qi Wang, Mitsuyoshi Hashimoto, Lei Shen, Shin Kawasaki, Hui Jung Kim, Tadashi Kohyama, Stephen I. Rennard,