Lipid modification gives rise to two distinct Haloferax volcanii S-layer glycoprotein populations

► The S-layer glycoprotein solely comprises the Haloferax volcanii surface layer ► Sequential EDTA and Triton X-100 treatment expose two distinct forms of the protein ► Both S-layer glycoprotein forms undergo signal peptide cleavage and N-glycosylation ► Only EDTA-solubilized S-layer glycoprotein is lipid-modified.