| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10797168 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2013 | 43 Pages |
Abstract
⺠Two novel antimicrobial peptides (AMPs) active against Gram â and Gram + bacteria ⺠Arg instead of Lys residues improve AMP activity against Gram + bacteria. ⺠AMP mode of action against Gram + requires electrostatic binding but also deeply insertion in the membrane. ⺠Lys vs Arg residues relevant for AMP specificity vs broad spectra of action
Keywords
AFMPoPCHC50RRMsLTAATCCLC50K. pneumoniaeMBCREEsRBCRP-HPLCMICIC50POPGred-edge excitation shiftE. coliPBSNaClAMPHEPESPBMCDi-8-ANEPPSLPS1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidE. faeciumMHbt1/2Staphylococcus aureusS. aureusStreptococcus pneumonialipoteichoic acidEscherichia coliEnterococcus faeciumlarge unilamellar vesiclespeptide–membrane interactionMinimal bactericidal concentrationMinimal inhibitory concentrationcircular dichroismSodium chloridePeripheral blood mononuclear cellbacterial shapeModel membranePhosphate buffered salineLUVlipopolysaccharideAmerican Type Culture CollectionMueller Hinton Brothatomic force microscopyKlebsiella pneumoniaAntimicrobial peptideH-bondHydrogen bondoptical densitycarboxyfluoresceinReversed phase high-performance liquid chromatographyred blood cell
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Inês M. Torcato, Yen-Hua Huang, Henri G. Franquelim, Diana Gaspar, David J. Craik, Miguel A.R.B. Castanho, Sónia Troeira Henriques,