| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10797181 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2013 | 11 Pages |
Abstract
⺠The native pI of membrane protein complexes is difficult to analyse ⺠The native pI has functional implications ⺠Membrane protein complexes for mitochondria and chloroplasts were analysed ⺠Native pIs were calculated for 2 membrane protein complexes and ATP-synthase F1 subcomplexes with improved accuracy ⺠Native pIs were also determined experimentally by liquid phase isoelectric focusing
Keywords
CMCSPADNSAMPSOLHCIINPIICSPAGEIEFTOFOPMFFEOXPHOSHPMCESIHEPESPDB4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acidblue-nativefree-flow electrophoresisFree flow electrophoresispolyacrylamide gel electrophoresisisoelectric focussingtime of flightMass-spectrometryCritical micellar concentrationOxidative phosphorylationLight-harvesting complex IImembrane protein complexesMitochondriaIsoelectric pointProtein Data Bankliquid chromatographyChloroplasts
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Authors
Christof Behrens, Kristina Hartmann, Stephanie Sunderhaus, Hans-Peter Braun, Holger Eubel,