NMR solution structure of C2 domain of MFG-E8 and insights into its molecular recognition with phosphatidylserine

► NMR solution structure of MFG-E8 C2 domain ► Electrostatic potential map of the C2 shows the clustered positive charges in the PS-binding site. ► K45, R79, and R146 residues of C2 are involved in the PS interaction. ► Mutation of the positively charged residues results in a dramatic decrease in the C2's PS binding.