Flexibility of the PDZ-binding motif in the micelle-bound form of Jagged-1 cytoplasmic tail

► Jagged-1 cytoplasmic tail binds to negatively charged micelles and liposomes. ► Binding is associated with the formation of four helical regions, as mapped by NMR. ► NMR shows that the C-terminal PDZ binding motif remains highly flexible. ► Phoshorylation abolishes the helical propensity in the C-terminal region. ► PDZ recognition may be tuned by phosphorylation upstream of the PDZ binding motif.