Lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes

Article ID	Journal	Published Year	Pages	File Type
10797471	Biochimica et Biophysica Acta (BBA) - Biomembranes	2012	10 Pages	PDF

Abstract

âº Membrane mimicking additives promote cell-free synthesis of soluble membrane proteins. âº Lipid-protein nanodiscs were compared with detergent micelles, bicelles and liposomes. âº Nanodiscs facilitate the correct folding of synthesized membrane proteins. âº Partial disintegration of nanodiscs composed of unsaturated lipids was observed. âº NMR studies of proteins co-translationally incorporated into nanodiscs are possible.

Keywords

LPN DTSP n-dodecyl-N,N-dimethylamine-N-oxide feeding mixture Brij-35 Tween-20 Nanodisc LDAO DMPG DOPG DPC PoPC MSP VSD SDS dHPC ESR IMP dMPC RST HSQC 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine 1,2-Dimyristoyl-sn-glycero-3-phosphoglycerol 1,2-dimyristoyl-sn-glycero-3-phosphocholine 1,2-dioleoyl-sn-glycero-3-phosphoglycerol 1,2-diheptanoyl-sn-glycero-3-phosphocholine 1,2-dihexanoyl-sn-glycero-3-phosphocholine Cell-free Cell-free expression Protein folding TROSY Triton X-100 NMR sodium dodecyl sulfate transverse relaxation-optimized spectroscopy transmembrane phosphatidylcholine reaction mixture Membrane mimetics Heteronuclear Single Quantum Correlation membrane scaffold protein Membrane protein Integral membrane protein