The role of endocytosis on the uptake kinetics of luciferin-conjugated cell-penetrating peptides

Article ID	Journal	Published Year	Pages	File Type
10797500	Biochimica et Biophysica Acta (BBA) - Biomembranes	2012	10 Pages	PDF

Abstract

âº Endocytosis inhibitors affect strongly the cytosolic delivery kinetics of CPPs. âº Luciferin-CPP conjugates are delivered into cytosol mainly via macropinocytosis. âº Involvement of other routes depends on the particular conjugate and its concentration. âº Kinetic analysis reveals clearly the concentration dependent internalization routes.

Keywords

HOBt N,N′-diisopropylethylamine short interfering ribonucleic acid t-BOC SPPS DIEA CyD tert-butyloxycarbonyl TBTU Macropinocytosis RLU NYS EGFR DCC CPZ DMF EGF CPP AUC N,N′-dicyclohexylcarbodiimide N,N′-dimethylformamide siRNA CME Endocytosis Clathrin-mediated endocytosis Bioluminescence Solid phase peptide synthesis cytochalasin D Uptake kinetics epidermal growth factor lactate dehydrogenase LDH Uptake mechanism area-under-curve Nystatin relative luminescence unit Cell-penetrating peptide Chloroquine chlorpromazine Epidermal growth factor receptor