| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10797542 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2012 | 10 Pages |
Abstract
⺠Heterologous production, purification of functional cytochrome b561-A and B-paralogs. ⺠Characterisation of a novel and atypical CYBASC protein from Arabidopsis thaliana. ⺠Determination of ascorbate oxidation and Fe3 +-chelates reductase activities. ⺠Determination of the haem midpoint potential (Em) values of the paralogs. ⺠Effects on Em of inhibitor allow assignment of ascorbate-binding site close to haem bL.
Keywords
SIIIMACBCAIEFDTTIPTGMNNGORFDEPCNaClBMGYBMMYCytochrome b561RCAESITMPDYNBBSADMSOn-Dodecyl-β-D-maltosideN-methyl-N′-nitro-N-nitrosoguanidineArabidopsis thalianabovine serum albuminSDS-PAGEsodium dodecyl sulphate polyacrylamide gel electrophoresisisopropyl β-D-1-thiogalactopyranosidebuffered methanol-complex mediumMdhabicinchoninic acidisoelectric focusingHeterologous productionBiochemical characterisationdaltondiethyl pyrocarbonateDimethyl sulfoxideZea maysSaccharomyces cerevisiaecolumn volumesMass spectrometryopen reading frameLuria-Bertanibuffered glycerol-complex mediumMolecular weightMembrane proteinImmobilised metal affinity chromatographyelectrospray ionisation
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Authors
Lucia Cenacchi, Manuela Busch, Philipp G. Schleidt, Florian G. Müller, Tina V.M. Stumpp, Werner Mäntele, Paolo Trost, C. Roy D. Lancaster,