Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10797572 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2012 | 12 Pages |
Abstract
⺠Human AQP8 forms a separate subfamily distinct from the other mammalian AQPs. ⺠Functional human AQP8 is overexpressed in P. pastoris at 2 mg/mL per liter of culture. ⺠Optimal solubilization is achieved with the detergent decyl maltoside. ⺠Removal of affinity tag yields highly ordered crystal arrays diffracting to 3 Ã
.
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Authors
Maria Agemark, Julia Kowal, Wanda Kukulski, Kristina Nordén, Niklas Gustavsson, Urban Johanson, Andreas Engel, Per Kjellbom,