| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10797629 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2011 | 8 Pages |
Abstract
⺠3 different AcrB variants with DARPins were analyzed via LILBID-MS, SEC and AUC. ⺠AcrB/DARPin complex stoichiometry differs depending on the AcrB variant used. ⺠AcrB trimerization is due to strong monomer interaction via the periplasmic loop. ⺠DARPin binding is stronger than the interaction between the AcrB monomers. ⺠A 3.1 kDa mass attached to the AcrB monomer might represent detergent and/or lipids.
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Authors
Lorenz Brandstätter, Lucie Sokolova, Thomas Eicher, Markus A. Seeger, Christophe Briand, Hi-jea Cha, Mihaela Cernescu, Jürgen Bohnert, Winfried V. Kern, Bernd Brutschy, Klaas M. Pos,