Pulmonary surfactant proteins and polymer combinations reduce surfactant inhibition by serum

► Addition of both SP-B and SP-C to lipids were better than adding either alone. ► Addition of any single polymer improved resistance to inhibition. ► Mixtures with SP-B and SP-C with combined polymers were more resistant to inactivation than native surfactant. ► Simple lipid mixtures work well with non-ionic polymers. ► Complex lipid mixtures work better with HA or HA combined with PEG or dextran.