Effects of positively charged arginine residues on membrane pore forming activity of Rev-NIS peptide in bacterial cells

Article ID	Journal	Published Year	Pages	File Type
10797676	Biochimica et Biophysica Acta (BBA) - Biomembranes	2011	7 Pages	PDF

Abstract

âº We design two analogs of Rev-NIS, substituted with arginine and serine. âº We investigate the antibacterial property of the peptides. âº All the peptides exert the activity on the bacterial membranes. âº Anal R is especially thought to be the most potent antibacterial peptide. âº The net positive charge of arginine residues is one of the most influential factors.

Keywords

SDS Rev protein 3,3′-dipropylthiadicarbocyanine iodide FITC-dextran Fluorenylmethyloxycarbonyl TFE CLSI FMOC HOBt TFA MIC DIC DMF FITC AMP DCM 1-hydroxybenzotriazole 1,3-diisopropylcarbodiimide 2,2,2-trifluoroethanol NiS N,N-dimethylformamide Analog Trifluoroacetic acid Indium tin oxide ITO ARPA Minimum inhibitory concentration Dichloromethane circular dichroism sodium dodecyl sulfate phosphatidylglycerol phosphatidylethanolamine fluorescein isothiocyanate Clinical and Laboratory Standards Institute HIV-1 Antibacterial peptide Antimicrobial peptide