Article ID Journal Published Year Pages File Type
10797706 Biochimica et Biophysica Acta (BBA) - Biomembranes 2011 10 Pages PDF
Abstract
► A new formalism to measure membrane partition coefficients using FCS is presented. ► Proteins are assumed to distribute among the liposomes according to a Poisson law. ► Presence of vestigial free dye causes an apparent maximal protein bound fraction lower than 100%. ► Lysozyme binding to POPS-containing liposomes is dominantly driven by electrostatic interactions. ► FCS measurements are easily uncoupled from potential interfering protein-mediated liposome aggregation.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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