Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10797706 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2011 | 10 Pages |
Abstract
⺠A new formalism to measure membrane partition coefficients using FCS is presented. ⺠Proteins are assumed to distribute among the liposomes according to a Poisson law. ⺠Presence of vestigial free dye causes an apparent maximal protein bound fraction lower than 100%. ⺠Lysozyme binding to POPS-containing liposomes is dominantly driven by electrostatic interactions. ⺠FCS measurements are easily uncoupled from potential interfering protein-mediated liposome aggregation.
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Authors
Ana M. Melo, Manuel Prieto, Ana Coutinho,