Molecular analysis of antimicrobial agent translocation through the membrane porin BpsOmp38 from an ultraresistant Burkholderia pseudomallei strain

► BpsOmp38 of B. pseudomallei was reconstituted into lipid bilayers for single-channel recordings. ► The trimeric BpsOmp38 formed an ion channel with the conductance of 2.7 ± 0.3 nS in 1 M KCl. ► Flux of seven antibiotics through BpsOmp38 was analyzed and translocation on/off rates were assessed. ► The dwell time of the antibiotic ertapenem decayed exponentially with increases in temperature. ► Energy barriers obtained from Arrhenius plots suggested one affinity site inside the Omp pores.