Article ID Journal Published Year Pages File Type
10798023 Biochimica et Biophysica Acta (BBA) - Biomembranes 2011 7 Pages PDF
Abstract
► The time course of Rb+ occlusion in gastric H+/K+-ATPase was measured at 25 °C. ► Rb+ occlusion was detectable only in the presence of vanadate. ► The enzyme-vanadate complex holding occluded Rb+ is very stable. ► A fraction of the bound Rb+ might not be occluded even in media with vanadate. ► Alamethicin showed to be a suitable permeabilizing agent for gastric vesicles.
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