Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10798023 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2011 | 7 Pages |
Abstract
⺠The time course of Rb+ occlusion in gastric H+/K+-ATPase was measured at 25 °C. ⺠Rb+ occlusion was detectable only in the presence of vanadate. ⺠The enzyme-vanadate complex holding occluded Rb+ is very stable. ⺠A fraction of the bound Rb+ might not be occluded even in media with vanadate. ⺠Alamethicin showed to be a suitable permeabilizing agent for gastric vesicles.
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Authors
Mónica R. Montes, Alejandro J. Spiaggi, José L.E. Monti, Flemming Cornelius, Claus Olesen, Patricio J. Garrahan, Rolando C. Rossi,