Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10798965 | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms | 2016 | 38 Pages |
Abstract
Nonsense-mediated mRNA decay (NMD) modulates the level of mRNA harboring a premature termination codon (PTC) in a translation-dependent manner. Inhibition of translation is known to impair NMD; however, few studies have investigated the correlation between enhanced translation and increased NMD. Here, we demonstrate that insulin signaling events increase translation, leading to an increase in NMD of eIF4E-bound transcripts. We provide evidence that (i) insulin-mediated enhancement of translation augments NMD and rapamycin abrogates this enhancement; (ii) an increase in AKT phosphorylation due to inhibition of PTEN facilitates NMD; (iii) insulin stimulation increases the binding of up-frameshift factor 1 (UPF1), most likely to eIF4E-bound PTC-containing transcripts; and (iv) insulin stimulation induces the colocalization of UPF1 and eIF4E in processing bodies. These results illustrate how extracellular signaling promotes the removal of eIF4E-bound NMD targets.
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Authors
Jungyun Park, Seyoung Ahn, Aravinth K. Jayabalan, Takbum Ohn, Hyun Chul Koh, Jungwook Hwang,