Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10799347 | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression | 2005 | 6 Pages |
Abstract
Fengycin is a cyclic lipopeptidic antibiotic produced nonribosomally by Bacillus subtilis. A fengycin synthetase mutant of B. subtilis F29-3 was generated with Tn917lux, which contains a transposon inserted in a 7716-bp gene, fenD. The mutation can be genetically complemented by transforming a plasmid carrying a wild-type fenD, confirming the participation of the gene in fengycin synthesis. Sequencing and biochemical analysis reveal that this gene encodes an enzyme that includes two amino acid-activating modules, FenD1 and FenD2, which activate l-Tyr and l-Thr, the third and the fourth amino acids in fengycin, respectively.
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Authors
Tsuey-Pin Lin, Chyi-Liang Chen, Hui-Chuan Fu, Cheng-Yeu Wu, Guang-Huey Lin, Shih-Hao Huang, Li-Kwan Chang, Shih-Tung Liu,