HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes - The ultimate approach for intra- and extracellular superoxide detection

Methodologies and site-specific fluorescent probes described in this review can be suitably employed to delineate oxy radical dependent mechanisms in cells under physiological and pathological conditions. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.