| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10800583 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2013 | 11 Pages |
Abstract
⺠The catalytic acid/base residue in a glycoside hydrolase family 3 β-glucosidase was identified. ⺠Site-directed mutagenesis, enzyme kinetic analysis, and nucleophile rescue were used. ⺠Prediction of the corresponding residue among other GH3 enzymes is enabled. ⺠These results are crucial to understanding catalysis and active-site structure in GH3.
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Authors
Preeyanuch Thongpoo, Lauren S. McKee, Ana Catarina Araújo, Prachumporn T. Kongsaeree, Harry Brumer,