Factors implicated in the assessment of aminolevulinic acid-induced protoporphyrin IX fluorescence

Article ID	Journal	Published Year	Pages	File Type
10800584	Biochimica et Biophysica Acta (BBA) - General Subjects	2013	13 Pages	PDF

Abstract

âº Analysis of ALA-induced PpIX fluorescence in co-cultures of tumor and normal esophageal cell lines âº Comparable ALA-induced PpIX fluorescence intensities in tumor and normal cell lines âº Heterogeneous mitochondrial activity throughout the monolayers of tumor and normal esophageal cell lines âº Positive collagen immunostaining in all tumor and normal esophageal cell lines tested

Keywords

ECM CMFDA 5-chloromethylfluorescein diacetate JC-1 ALA SSC PDT PPIX FSC NaOH FBS PBS 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide DMSO EDTA Ethylenediaminetetraacetic acid δ-aminolevulinic acid Aminolevulinic acid Tris–HCl Photodynamic therapy Dimethylsulfoxid fetal bovine serum Phosphate buffered saline Extracellular matrix Mitochondrial potential forward scatter side scatter protoporphyrin IX Collagen