| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10800634 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2013 | 8 Pages |
Abstract
⺠The reduction of tissue factor (TF) disulfides abolishes TF cofactor function. ⺠Reduced TF binds to factor VIIa but does not increase factor VIIa activity. ⺠Treatment of reduced TF with PDI does not restore TF function.
Keywords
PTFGSSGCysPCPsHRPGSHTMBDTTFVIIFVIIaIAAmAbFIUfluorescence intensity unitsLPSPhosphotidylcholinePDI3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonateBSAbovine serum albuminMonoclonal antibodyethylenediaminetetraacetic acid disodium salt dihydrateEDTAimmunoglobulin tetramethylbenzidineFluorogenic assayDisulfidesdithiothreitolCysteineMass-spectrometryMass spectrometryTissue factorFactor VIIFactor VIIaFactor XPhosphatidylserinelipopolysaccharidehorse radish peroxidaseprotein disulfide isomerasepolyethylene glycolPEGCHAPSreduced glutathioneliquid chromatographyoxidized glutathioneiodoacetamide
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Authors
Jolanta Krudysz-Amblo, Mark E. II, Tyler Knight, Dwight E. Matthews, Kenneth G. Mann, Saulius Butenas,