| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10800635 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2013 | 12 Pages |
Abstract
⺠Screening approach to prioritize integral membrane proteins for structural studies ⺠Rapid and cost efficient screening strategy for all IMPs regardless of topology ⺠Automated analytical gel filtration as quality control tool ⺠Strategy validation by analysis of 48 different IMPs
Keywords
isopropyl-ß-d-thiogalactopyranosideIMPE. coliGPAAGFIMACHRPLICDTTIPTGGFPCYMAL-5Detergent screenTRISDDMSECLDAOFSECbisTrisBSAdodecyl-β-D-maltosideN,N-dimethyldodecylamine-N-oxideSDS–PAGEtris(2-carboxyethyl)phosphinebovine serum albuminEDTAEthylenediaminetetraacetic acidEscherichia coliSodium dodecyl sulfate polyacrylamide gel electrophoresisSize-exclusion chromatographyBassTevoptical density at 600 nmPotdithiothreitolMateMembrane protein structureTCEPterrific brothLigation-independent cloninggel filtrationLuria BertaniMultidrug And Toxic compound ExtrusionTobacco etch virusHorseradish peroxidaseIntegral membrane proteingreen fluorescent proteinStructural genomicsGlycophorin A
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Authors
Christian Löw, Per Moberg, Esben M. Quistgaard, Marie Hedrén, Fatma Guettou, Jens Frauenfeld, Lars Haneskog, Pär Nordlund,