| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10800709 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2012 | 6 Pages |
Abstract
⺠We determined the crystal structure of Delta-class GST from Bombyx mori (bmGSTD). ⺠Secondary and tertiary structures are highly conserved in bmGSTD. ⺠bmGSTD is stabilized by a hydrophobic lock-and-key motif. ⺠We determined crucial residues for glutathione binding by site-directed mutagenesis. ⺠Our study reveals possible catalysis mechanisms in glutathione conjugation of bmGSTD.
Keywords
GSHCoomassie Brilliant Blue R250S-hexyl glutathioneGTXCDNBCBBRT-PCRGSTIPTGECA4-hydroxynonenal4-HNE1-chloro-2,4-dinitrobenzeneSite-directed mutagenesisReverse transcriptase PCRROSEthacrynic acidisopropyl 1-thio-β-d-galactosideLuria–Bertani mediumCrystal structurepolymerase chain reactionPCRLepidopterapolyethylene glycolPEGGlutathioneGlutathione transferaseReactive oxygen species
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Authors
Kohji Yamamoto, Kazuhiro Usuda, Yoshimitsu Kakuta, Makoto Kimura, Akifumi Higashiura, Atsushi Nakagawa, Yoichi Aso, Mamoru Suzuki,