| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10800763 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2012 | 5 Pages |
Abstract
⺠We measure high-pressure Trp-fluorescence spectra of staphylococcal nuclease (SNase). ⺠We examine three SNase samples in the presence of crowding agents. ⺠All the samples exhibit a two-state behavior (native and unfolded states). ⺠We find SNase unfolding as a function of pressure is impeded in crowded solutions. ⺠The result suggests this crowding effect may also apply to the proteins in cells.
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Authors
Suntao Wang, Mark W. Tate, Sol M. Gruner,