Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10800970 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2008 | 7 Pages |
Abstract
The conversion of fatty acid hydroperoxides to allene epoxides is catalysed by a cytochrome P450 in plants. In contrast, in the coral Plexaura homomalla, a catalase-related hemoprotein fused to the lipoxygenase (LOX) was found to function as an allene oxide synthase. This work reports the homology-based RT-PCR cloning and functional expression of a Gersemia fruticosa analogue of the allene oxide synthase-lipoxygenase (AOS-LOX) fusion protein. The G. fruticosa mRNA codes for a protein with 84% sequence identity to the P. homomalla AOS-LOX. Our data indicate that the AOS-LOX fusion protein pathway is used by another coral and P. homomalla represents no exception.
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Authors
Helike Lõhelaid, Reet Järving, Karin Valmsen, Külliki Varvas, Malle Kreen, Ivar Järving, Nigulas Samel,