Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10801057 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2005 | 11 Pages |
Abstract
The final S. anginosus integrant successfully secreted the enzymatically active gtfI gene products and extracellular enzyme was characterized. This enzyme produced water-insoluble glucans and glucan-forming activity was stimulated by the addition of dextranT10. When this integrant was grown in Todd-Hewitt broth supplemented with sucrose, the integrant adhered to the glass surface in vitro and this integrant exhibited the different colony morphology on Mitis-Salivarius agar plates compared to S. sobrinus and S. anginosus. These observations strongly suggest that the construction of S. anginosus integrant expressing S. sobrinus gtfI gene using this transformation system may be an effective means of analysis of cariogenic biofilm formation.
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Authors
Noriko Shinozaki-Kuwahara, Teruaki Shiroza, Mitsuo Hayakawa, Yoshimitsu Abiko, Kazuo Fukushima,