Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10801731 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2016 | 24 Pages |
Abstract
In contrast, in IMR-32 NB cells, the same concentrations of TTFA markedly suppressed cisplatin-induced apoptosis. Comparison of oxygen consumption in cisplatin-resistant SK-N-BE(2) and cisplatin-sensitive IMR-32 cells clearly demonstrated impaired Complex II activity in IMR-32 cells. We also found that in SK-N-BE(2) cells co-treatment with cisplatin and TTFA markedly stimulated formation of reactive oxygen species (ROS), whereas in IMR cells, cisplatin-mediated ROS production was attenuated by TTFA, which explains apoptosis suppression in these cells. Thus, functionally active SDH is a prerequisite for the ROS-mediated sensitization to treatment by TTFA.
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Authors
Björn Kruspig, Kadri Valter, Belma Skender, Boris Zhivotovsky, Vladimir Gogvadze,