Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10801754 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2016 | 9 Pages |
Abstract
Here we show that Pnc1 physically interacts with Gpd1, which is required for piggy-back import of Pnc1 into peroxisomes. Quantitative fluorescence microscopy analyses revealed that the levels of both proteins increased in peroxisomes and in the cytosol upon exposure of cells to stress. However, upon exposure of cells to stress we also observed enhanced cytosolic levels of the control PTS2 protein thiolase, when produced under control of the GPD1 promoter. This suggests that these conditions cause a partial defect in PTS2 protein import, probably because the PTS2 import pathway is easily saturated.
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Authors
Sanjeev Kumar, Ritika Singh, Chris P. Williams, Ida J. van der Klei,