Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10801981 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2015 | 14 Pages |
Abstract
Brief changes in the cytosolic and intra-organellar Ca2Â + concentration serve as specific signals for various physiological processes. In mesothelial cells lining the surface of internal organs and the walls of body cavities, a re-entry in the cell cycle (G0-G1 transition) evoked by serum re-administration induces long-lasting Ca2Â + oscillations with a slowly decreasing frequency. Individual mesothelial cells show a wide range of different oscillatory patterns within a single, supposedly homogenous cell population. Changes in the cytoplasmic Ca2Â + concentration (ccyt) show baseline oscillatory patterns i.e., discrete Ca2Â + transients starting from a constant basal ccyt level. The ER Ca2Â + concentration (cER) displays a sawtooth wave at a semi-depleted ER state; the minimum level is reached just briefly after the maximal value for ccyt. These oscillations depend on plasmalemmal Ca2Â + influx and on the inositol trisphosphate concentration [InsP3]; the Ca2Â + influx is a crucial determinant of the oscillation frequency. Partial blocking of SERCA pumps modifies the oscillation frequency in both directions, i.e. increasing it in some cells and lowering it in others. Current mathematical models for Ca2Â + oscillations mostly fail to reproduce two experimentally observed phenomena: the broad range of interspike intervals and constant basal ccyt levels between two Ca2Â + spikes. Here we developed a new model based on - and fitted to - Ca2Â + recordings of ccyt and cER recorded in primary mouse mesothelial cells. The model allowed for explaining many features of experimentally observed Ca2Â + oscillations. We consider this model to be suitable to simulate various types of InsP3 receptor-based baseline Ca2Â + oscillations.
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Authors
László Pecze, Beat Schwaller,