Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid encoding interleukin-10

In the present study, we investigated MSO10H6MSO10H6 as a carrier system to introduce a plasmid encoding murine interleukin-10 (pIL-10) to modulate alloreactivity of dendritic cells (DC). Results indicate that MSO10H6MSO10H6 formed stable and protective nano-sized particles with pIL-10. Gene-modified DC elicited weak proliferation of allogeneic CD4 and CD8 T cells in vitro. Using cell-embedded Matrigel as a surrogate graft, we also showed that DC transfected with MSO10H6MSO10H6 complexed with pIL-10 suppressed host cell infiltration in vivo. These data demonstrate that the self-assembled system of MSO10H6MSO10H6 is an effectual delivery vehicle for plasmid-based modulation of DC-dependent allogeneic T cell responses.