Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10802 | Biomaterials | 2006 | 7 Pages |
Abstract
In the present study, we investigated MSO10H6MSO10H6 as a carrier system to introduce a plasmid encoding murine interleukin-10 (pIL-10) to modulate alloreactivity of dendritic cells (DC). Results indicate that MSO10H6MSO10H6 formed stable and protective nano-sized particles with pIL-10. Gene-modified DC elicited weak proliferation of allogeneic CD4 and CD8 T cells in vitro. Using cell-embedded Matrigel as a surrogate graft, we also showed that DC transfected with MSO10H6MSO10H6 complexed with pIL-10 suppressed host cell infiltration in vivo. These data demonstrate that the self-assembled system of MSO10H6MSO10H6 is an effectual delivery vehicle for plasmid-based modulation of DC-dependent allogeneic T cell responses.
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Authors
Liang Jia, Jeffrey R. Kovacs, Ying Zheng, Ellen S. Gawalt, Hongmei Shen, Wilson S. Meng,